The Core Research Assistant II carries out the day-to-day flow of the lab under the direction of the Core Manager or Director. Under minimal supervision, the Core Research Assistant II will execute genetic protocols including nucleic acid quantification, DNA/RNA next-generation library builds and high throughput sequencing. Exercise discretion and independent judgment to optimize protocols and electronically track collected samples and data generated. Present and explain results to Core customers under manager's supervision. Assist in other core laboratory and research functions, which may include appropriately documenting and billing services provided.
Key Functions and Expected Performances:
- Under minimal supervision, plan, conduct and evaluate experiments and assays of varying complexity
- Electronically track collected samples
- Understand, optimize, and follow experimental protocols
- Record, compile, track, and report data
- Analyze and interpret data
- Assist in workflow design and problem solving
- Provide training to new staff
- Ensure laboratory is prepared for experiments
- Maintain inventories and order reagents
- Maintain sterile conditions as required
- Clean equipment
- Maintain the instrument/equipment used for services provided by the core
- Read and follow all equipment manuals
- Maintain and repair equipment according to established guidelines
- Provide training to researchers in instrument/equipment operation
- Troubleshoots instrument/equipment problems
- May utilize the iLAB system for documenting services provided and to ensure services are appropriately billed to clients
- Accurately compile information for monthly billing.
This position requires a Bachelors degree in a scientifically related field and laboratory experience in molecular techniques such as DNA/RNA extraction and quantification, conventional polymerase chain reaction (PCR), quantitative real-time PCR or other nucleic acid manipulation. Next-generation sequencing experience is a plus. Facility with both spoken and written English is necessary.
Bachelor's Degree (or equivalent experience) and 2 years experience
Licensure, Certification, and/or Registration (LCR):
- Occasional: Sitting: Remaining in seated position
- Occasional: Standing: Remaining on one's feet without moving.
- Occasional: Walking: Moving about on foot.
- Occasional: Lifting under 35 lbs: Raising and lowering objects under 35 lbs from one level to another
- Occasional: Push/Pull: Exerting force to move objects away from or toward.
- Occasional: Bending/Stooping: Trunk bending downward and forward by bending spine at waist requiring full use of lower extremities and back muscles
- Occasional: Reaching below shoulders: Extending arms in any direction below shoulders.
- Occasional: Handling: Seizing, holding, grasping, turning or otherwise working with hand or hands.
- Occasional: Vision: Clarity of near vision at 20 inches or less and far vision at 20 feet or more with depth perception, peripheral vision, color vision.
- Frequent: Noise: May include exposure to occupational noise levels which equal or exceed an 8-hr time-weighted average of 85 decibels, requiring enrollment in VUMC's Hearing Conservation Program which includes training, use of hearing protection, and periodic audiometry.
- Continuous: Communication: Expressing or exchanging written/verbal/electronic information.
Physical Requirements/Strengths needed & Physical Demands:
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